Review



nco i/ xho i sites of pet32b(+) plasmid  (Merck KGaA)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 90
      Buy from Supplier

    Structured Review

    Merck KGaA nco i/ xho i sites of pet32b(+) plasmid
    Nco I/ Xho I Sites Of Pet32b(+) Plasmid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nco i/ xho i sites of pet32b(+) plasmid/product/Merck KGaA
    Average 90 stars, based on 1 article reviews
    nco i/ xho i sites of pet32b(+) plasmid - by Bioz Stars, 2026-02
    90/100 stars

    Images



    Similar Products

    plasmid pet32b-hrv3c  (Eurofins)
    90
    Eurofins plasmid pet32b-hrv3c
    Plasmid Pet32b Hrv3c, supplied by Eurofins, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pet32b-hrv3c/product/Eurofins
    Average 90 stars, based on 1 article reviews
    plasmid pet32b-hrv3c - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    modified pet32b-α 3 c plasmid  (GenScript corporation)
    90
    GenScript corporation modified pet32b-α 3 c plasmid
    Modified Pet32b α 3 C Plasmid, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/modified pet32b-α 3 c plasmid/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    modified pet32b-α 3 c plasmid - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    plasmid pet32b  (GenScript corporation)
    90
    GenScript corporation plasmid pet32b
    Plasmid Pet32b, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pet32b/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    plasmid pet32b - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    pet32b-based plasmid  (Millipore)
    90
    Millipore pet32b-based plasmid
    Pet32b Based Plasmid, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet32b-based plasmid/product/Millipore
    Average 90 stars, based on 1 article reviews
    pet32b-based plasmid - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    plasmid pet32b  (Millipore)
    90
    Millipore plasmid pet32b
    Plasmid Pet32b, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pet32b/product/Millipore
    Average 90 stars, based on 1 article reviews
    plasmid pet32b - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    pet32b plasmid  (TaKaRa)
    86
    TaKaRa pet32b plasmid
    Scheme of the recombinant proteins as produced upon cloning in <t>pET32b</t> (Trx-TB10.4, Trx-Ag85B and Trx- full ) or pColdI (His- full 1 and His- full 2) vectors. Trx: thioredoxin (116 amino acids, aa). The arrow indicates the EK cleavage site. The entire amino acid sequences are reported in the Additional file : Figure S2.
    Pet32b Plasmid, supplied by TaKaRa, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pet32b plasmid/product/TaKaRa
    Average 86 stars, based on 1 article reviews
    pet32b plasmid - by Bioz Stars, 2026-02
    86/100 stars
    		  Buy from Supplier
    nco i/ xho i sites of pet32b(+) plasmid  (Merck KGaA)
    90
    Merck KGaA nco i/ xho i sites of pet32b(+) plasmid
    Scheme of the recombinant proteins as produced upon cloning in <t>pET32b</t> (Trx-TB10.4, Trx-Ag85B and Trx- full ) or pColdI (His- full 1 and His- full 2) vectors. Trx: thioredoxin (116 amino acids, aa). The arrow indicates the EK cleavage site. The entire amino acid sequences are reported in the Additional file : Figure S2.
    Nco I/ Xho I Sites Of Pet32b(+) Plasmid, supplied by Merck KGaA, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/nco i/ xho i sites of pet32b(+) plasmid/product/Merck KGaA
    Average 90 stars, based on 1 article reviews
    nco i/ xho i sites of pet32b(+) plasmid - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    recombinant plasmid pet32b-trxa-apafp752  (Promega)
    90
    Promega recombinant plasmid pet32b-trxa-apafp752
    Scheme of the recombinant proteins as produced upon cloning in <t>pET32b</t> (Trx-TB10.4, Trx-Ag85B and Trx- full ) or pColdI (His- full 1 and His- full 2) vectors. Trx: thioredoxin (116 amino acids, aa). The arrow indicates the EK cleavage site. The entire amino acid sequences are reported in the Additional file : Figure S2.
    Recombinant Plasmid Pet32b Trxa Apafp752, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant plasmid pet32b-trxa-apafp752/product/Promega
    Average 90 stars, based on 1 article reviews
    recombinant plasmid pet32b-trxa-apafp752 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier
    plasmid pet32b-ncr169  (GenScript corporation)
    90
    GenScript corporation plasmid pet32b-ncr169
    Scheme of the recombinant proteins as produced upon cloning in <t>pET32b</t> (Trx-TB10.4, Trx-Ag85B and Trx- full ) or pColdI (His- full 1 and His- full 2) vectors. Trx: thioredoxin (116 amino acids, aa). The arrow indicates the EK cleavage site. The entire amino acid sequences are reported in the Additional file : Figure S2.
    Plasmid Pet32b Ncr169, supplied by GenScript corporation, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid pet32b-ncr169/product/GenScript corporation
    Average 90 stars, based on 1 article reviews
    plasmid pet32b-ncr169 - by Bioz Stars, 2026-02
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Scheme of the recombinant proteins as produced upon cloning in pET32b (Trx-TB10.4, Trx-Ag85B and Trx- full ) or pColdI (His- full 1 and His- full 2) vectors. Trx: thioredoxin (116 amino acids, aa). The arrow indicates the EK cleavage site. The entire amino acid sequences are reported in the Additional file : Figure S2.

    Journal: Microbial Cell Factories

    Article Title: Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins

    doi: 10.1186/1475-2859-12-115

    Figure Lengend Snippet: Scheme of the recombinant proteins as produced upon cloning in pET32b (Trx-TB10.4, Trx-Ag85B and Trx- full ) or pColdI (His- full 1 and His- full 2) vectors. Trx: thioredoxin (116 amino acids, aa). The arrow indicates the EK cleavage site. The entire amino acid sequences are reported in the Additional file : Figure S2.

    Article Snippet: To prepare His- full 1 form, the full cDNA was extracted from pET32b plasmid using Kpn I and Eco RI restriction sites and cloned into pColdI plasmid (TaKaRa Bio Inc., Otsu, Japan).

    Techniques: Recombinant, Produced, Clone Assay

    Time course of pH (○, solid line), dO 2 (♦, solid line) and OD 600nm (●, dashed line) in 3 L batch cultivation trials of recombinant E. coli BL21(DE3) cells producing M. tuberculosis immunogenic proteins. A) E. coli BL21(DE3) cells containing pET32b-Trx-TB10.4 plasmid and grown in LB medium. B) E. coli BL21(DE3) cells containing pET32b-Trx-Ag85B plasmid and grown in SB medium. C) E. coli BL21(DE3) cells containing pColdI-His- full 2 and growing in SB/NaCl medium. Temperature (■, dashed line) was kept constant at 37°C before IPTG addition and then reduced to 18°C for Trx-TB10.4 and Trx-Ag85B production, and at 15°C for His- full 2 production, until harvest. The induction of protein expression was done at OD 600nm = 0.8 with 0.1 mM IPTG for Trx-TB10.4, at OD 600nm = 2 with 0.1 mM IPTG for Trx-Ag85B, and at OD 600nm = 2 with 0.1 mM IPTG for His- full 2. Biomass (wet weight) collected at the harvest time was 8.5 g/L for Trx-TB10.4, 19.5 g/L for Trx-Ag85B and 6.5 g/L for His- full 2.

    Journal: Microbial Cell Factories

    Article Title: Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins

    doi: 10.1186/1475-2859-12-115

    Figure Lengend Snippet: Time course of pH (○, solid line), dO 2 (♦, solid line) and OD 600nm (●, dashed line) in 3 L batch cultivation trials of recombinant E. coli BL21(DE3) cells producing M. tuberculosis immunogenic proteins. A) E. coli BL21(DE3) cells containing pET32b-Trx-TB10.4 plasmid and grown in LB medium. B) E. coli BL21(DE3) cells containing pET32b-Trx-Ag85B plasmid and grown in SB medium. C) E. coli BL21(DE3) cells containing pColdI-His- full 2 and growing in SB/NaCl medium. Temperature (■, dashed line) was kept constant at 37°C before IPTG addition and then reduced to 18°C for Trx-TB10.4 and Trx-Ag85B production, and at 15°C for His- full 2 production, until harvest. The induction of protein expression was done at OD 600nm = 0.8 with 0.1 mM IPTG for Trx-TB10.4, at OD 600nm = 2 with 0.1 mM IPTG for Trx-Ag85B, and at OD 600nm = 2 with 0.1 mM IPTG for His- full 2. Biomass (wet weight) collected at the harvest time was 8.5 g/L for Trx-TB10.4, 19.5 g/L for Trx-Ag85B and 6.5 g/L for His- full 2.

    Article Snippet: To prepare His- full 1 form, the full cDNA was extracted from pET32b plasmid using Kpn I and Eco RI restriction sites and cloned into pColdI plasmid (TaKaRa Bio Inc., Otsu, Japan).

    Techniques: Recombinant, Plasmid Preparation, Expressing

    Chemico-physical parameters of the recombinant proteins as produced upon cloning in pET32b or pColdI

    Journal: Microbial Cell Factories

    Article Title: Optimizing Escherichia coli as a protein expression platform to produce Mycobacterium tuberculosis immunogenic proteins

    doi: 10.1186/1475-2859-12-115

    Figure Lengend Snippet: Chemico-physical parameters of the recombinant proteins as produced upon cloning in pET32b or pColdI

    Article Snippet: To prepare His- full 1 form, the full cDNA was extracted from pET32b plasmid using Kpn I and Eco RI restriction sites and cloned into pColdI plasmid (TaKaRa Bio Inc., Otsu, Japan).

    Techniques: Recombinant, Produced, Clone Assay